Chip PCR. I. Surface passivation of microfabricated silicon-glass chips for PCR

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Chip PCR. I. Surface passivation of microfabricated silicon-glass chips for PCR.

The microreaction volumes of PCR chips (a microfabricated silicon chip bonded to a piece of flat glass to form a PCR reaction chamber) create a relatively high surface to volume ratio that increases the significance of the surface chemistry in the polymerase chain reaction (PCR). We investigated several surface passivations in an attempt to identify 'PCR friendly' surfaces and used those surfac...

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ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 1996

ISSN: 1362-4962

DOI: 10.1093/nar/24.2.375